rabbit polyclonal anti igf irα antibody  (Santa Cruz Biotechnology)

Journal: Frontiers in Cellular Neuroscience

Article Title: Bidirectional modulation of synaptic transmission by insulin-like growth factor-I

doi: 10.3389/fncel.2024.1390663

Figure Lengend Snippet: IGF-IRs are present at presynaptic sites of both excitatory and inhibitory synapses. Electron micrographs demonstrating IGF-IR silver enhanced immunogold labeling (white arrows) within both excitatory (A) and inhibitory (B) synapses. Immuno-particles were found associated to presynaptic terminals (A,B) . Asterisks indicate presynaptic terminals. Scale bars, 0.2 μm. Apposition of IGF-IR immunoreactive puncta (red) with both the postsynaptic marker PSD95 (green, white arrowheads in C ) and the presynaptic marker vGAT (green, white arrowheads in D ) agree with localization of IGF-IR at both sides of the synapse. DAPI staining of cell nuclei (blue).

Article Snippet: Following a standard immunocytochemical protocol, tissue was first free-floating incubated in a rabbit polyclonal anti-IGF-IRα antibody (1/250, Santa Cruz) in a PBS 0.1 M/1% BSA solution for 48 h at 22°C.

Techniques: Labeling, Marker, Staining

Journal: Journal of Signal Transduction

Article Title: Estrogen Regulates MAPK-Related Genes through Genomic and Nongenomic Interactions between IGF-I Receptor Tyrosine Kinase and Estrogen Receptor-Alpha Signaling Pathways in Human Uterine Leiomyoma Cells

doi: 10.1155/2012/204236

Figure Lengend Snippet: Differential MAPK pathway-related gene expression in UtLM cells mediated by 17 β -estradiol (E 2 ) in the presence of scrambled siRNA (siScr) or IGF-IR silencing (siIGF-IR). (a) Heat maps of real-time RT 2 Profiler PCR Array of MAPK-related genes. The red areas represent the genes that are upregulated, and the green areas represent the genes that are downregulated by E 2 treatment. (b) Plot of fold changes of MAPK-related genes in response to E 2 treatment in UtLM cells with siScr or siIGF-IR.

Article Snippet: Primary antibodies used for the western blotting were as follows: rabbit polyclonal anti-phospho-IGF-1R β Tyr1131/IR- β Tyr1146 (number 3021, Cell Signaling), rabbit polyclonal anti-IGF-IR β (sc-713, Santa Cruz Biotechnology), rabbit polyclonal anti-phospho-Shc (number 2434, Cell Signaling), rabbit polyclonal anti-Shc (sc-288, Santa Cruz), rabbit polyclonal anti-ER α (sc-7207, Santa Cruz), mouse monoclonal anti-phospho-ER α Ser118 (number 2511, Cell Signaling), rabbit polyclonal anti-MAPKp44/42, and rabbit polyclonal anti-phospho-MAPKp44/42 (number 9201 and number 9101, Cell Signaling).

Techniques: Expressing

Journal: Journal of Signal Transduction

Article Title: Estrogen Regulates MAPK-Related Genes through Genomic and Nongenomic Interactions between IGF-I Receptor Tyrosine Kinase and Estrogen Receptor-Alpha Signaling Pathways in Human Uterine Leiomyoma Cells

doi: 10.1155/2012/204236

Figure Lengend Snippet: Differential expression of phosphorylated (p)IGF-IR, pMAPKp44/42, and pER α ser118 in UtLM cells with scrambled siRNA (siScr) or IGF-IR silencing (siIGF-IR) followed by 17 β -estradiol (E 2 ) treatment. (a) Western blot of IGF-IR/ER α pathway proteins in UtLM cells. (b) Comparison of ratio of densitometric band intensities of phosphorylated (phospho)/total proteins in UtLM cells with siScr or siIGF-IR followed by E 2 treatment. Bars represent mean ± SEM of three independent experiments. * P < 0.05 versus 0 min.

Article Snippet: Primary antibodies used for the western blotting were as follows: rabbit polyclonal anti-phospho-IGF-1R β Tyr1131/IR- β Tyr1146 (number 3021, Cell Signaling), rabbit polyclonal anti-IGF-IR β (sc-713, Santa Cruz Biotechnology), rabbit polyclonal anti-phospho-Shc (number 2434, Cell Signaling), rabbit polyclonal anti-Shc (sc-288, Santa Cruz), rabbit polyclonal anti-ER α (sc-7207, Santa Cruz), mouse monoclonal anti-phospho-ER α Ser118 (number 2511, Cell Signaling), rabbit polyclonal anti-MAPKp44/42, and rabbit polyclonal anti-phospho-MAPKp44/42 (number 9201 and number 9101, Cell Signaling).

Techniques: Expressing, Western Blot

Journal: Journal of Signal Transduction

Article Title: Estrogen Regulates MAPK-Related Genes through Genomic and Nongenomic Interactions between IGF-I Receptor Tyrosine Kinase and Estrogen Receptor-Alpha Signaling Pathways in Human Uterine Leiomyoma Cells

doi: 10.1155/2012/204236

Figure Lengend Snippet: Increased immunoprecipitation of IGF-IR and Shc with ER α in cells exposed to 17 β -estradiol (E 2 ). (a) Interactions between IGF-IR β and Shc with ER α in UtLM cells were determined by immunoprecipitation (IP). (b) Comparison of densitometric band intensity of immunoblots (IB) in UtLM cells with siScr or siIGF-IR followed by E 2 treatment. Representative of three independent experiments. Bars represent mean intensities ± SEM. * P < 0.05 versus 0 min.

Article Snippet: Primary antibodies used for the western blotting were as follows: rabbit polyclonal anti-phospho-IGF-1R β Tyr1131/IR- β Tyr1146 (number 3021, Cell Signaling), rabbit polyclonal anti-IGF-IR β (sc-713, Santa Cruz Biotechnology), rabbit polyclonal anti-phospho-Shc (number 2434, Cell Signaling), rabbit polyclonal anti-Shc (sc-288, Santa Cruz), rabbit polyclonal anti-ER α (sc-7207, Santa Cruz), mouse monoclonal anti-phospho-ER α Ser118 (number 2511, Cell Signaling), rabbit polyclonal anti-MAPKp44/42, and rabbit polyclonal anti-phospho-MAPKp44/42 (number 9201 and number 9101, Cell Signaling).

Techniques: Immunoprecipitation, Western Blot

Journal: Journal of Signal Transduction

Article Title: Estrogen Regulates MAPK-Related Genes through Genomic and Nongenomic Interactions between IGF-I Receptor Tyrosine Kinase and Estrogen Receptor-Alpha Signaling Pathways in Human Uterine Leiomyoma Cells

doi: 10.1155/2012/204236

Figure Lengend Snippet: Schematic illustration of genomic and nongenomic actions of ER α on target gene transcription. Genomic actions involve the translocation of cytoplasmic E 2 -ER α complexes to the nucleus which can then bind directly to estrogen response elements (EREs) in target gene promoters or nuclear E 2 -ER α complexes. These complexes are tethered through protein-protein interactions to a transcription factor complex (TF) that contacts the target gene promoter to induce transcription of IGF-I and MAPK related genes. Nongenomically, E 2 can bind to membrane associated ER α which then binds to the adaptor protein, Src collagen homologue (Shc) to form a protein complex consisting of ER α and Shc and/or ER α and IGF-IR. E 2 signals through the IGF-IR and activates MAPKp44/42, which can then phosphorylate ER α at the serine118 site to initiate transcription (txn) of MAPK related genes. CCNDs = Cyclin Ds; MAPKs = mitogen-activated protein kinases; DLK1 = delta-like 1 homolog; COL1A1 = collagen type I alpha 1.

Article Snippet: Primary antibodies used for the western blotting were as follows: rabbit polyclonal anti-phospho-IGF-1R β Tyr1131/IR- β Tyr1146 (number 3021, Cell Signaling), rabbit polyclonal anti-IGF-IR β (sc-713, Santa Cruz Biotechnology), rabbit polyclonal anti-phospho-Shc (number 2434, Cell Signaling), rabbit polyclonal anti-Shc (sc-288, Santa Cruz), rabbit polyclonal anti-ER α (sc-7207, Santa Cruz), mouse monoclonal anti-phospho-ER α Ser118 (number 2511, Cell Signaling), rabbit polyclonal anti-MAPKp44/42, and rabbit polyclonal anti-phospho-MAPKp44/42 (number 9201 and number 9101, Cell Signaling).

Techniques: Translocation Assay

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Human placental GLUT1 glucose transporter expression and the fetal insulin-like growth factor axis in pregnancies complicated by diabetes

doi: 10.1016/j.bbadis.2019.06.002

Figure Lengend Snippet: GLUT1 expression on syncytial microvillous and basal membranes. A. Expression of GLUT1 protein, determined by slot-blotting, on microvillous membranes from control, GDM A2 and IDDM pregnancies (NS, ANOVA; n = 22, 38, 17). B. Expression of GLUT1 protein on basal membranes from control, GDM A2 and IDDM pregnancies (p < 0.05, ANOVA; n = 19, 24, 14).

Article Snippet: Membranes were then blocked with SuperBlock (Pierce Biotechnology, Rockford, IL) for 60 min and incubated with rabbit polyclonal anti-GLUT1 or anti-IGF-IR ß-subunit antibodies (1:20,000; EMD Millipore, Cat# 07–1401, 04–298) in TBS containing 3% BSA for 120 min. Specificity was assured by absence of a signal following omission of the primary antibody.

Techniques: Expressing

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Human placental GLUT1 glucose transporter expression and the fetal insulin-like growth factor axis in pregnancies complicated by diabetes

doi: 10.1016/j.bbadis.2019.06.002

Figure Lengend Snippet: Glucose transport activity of syncytial microvillous and basal membranes. A. Glucose transporter activity, determined by stopped-flow, of microvillous membranes from control, GDM A2 and IDDM pregnancies (NS, ANOVA; n = 9, 10, 10). B. Glucose transporter activity of basal membranes from control, GDM A2 and IDDM pregnancies (p < 0.05, ANOVA; n = 10, 8, 10). C. No-protein mediated diffusional transport activity of microvillous and basal membranes from control, GDM A2 and IDDM pregnancies (numbers as for Figure 4A).

Article Snippet: Membranes were then blocked with SuperBlock (Pierce Biotechnology, Rockford, IL) for 60 min and incubated with rabbit polyclonal anti-GLUT1 or anti-IGF-IR ß-subunit antibodies (1:20,000; EMD Millipore, Cat# 07–1401, 04–298) in TBS containing 3% BSA for 120 min. Specificity was assured by absence of a signal following omission of the primary antibody.

Techniques: Activity Assay

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Human placental GLUT1 glucose transporter expression and the fetal insulin-like growth factor axis in pregnancies complicated by diabetes

doi: 10.1016/j.bbadis.2019.06.002

Figure Lengend Snippet: Relationship between birth weight and basal membrane GLUT1 expression and glucose transporter activity. A. Birth weight plotted against GLUT1 expression in basal membranes from control, GDM A2 and IDDM pregnancies. The regression line is plotted for the control group only; no correlation was observed for the diabetic groups. B. Birth weight plotted against glucose transport activity for basal membranes from control, GDM A2 and IDDM pregnancies. The regression line is plotted for the control group; no correlation was observed for the diabetic groups.

Article Snippet: Membranes were then blocked with SuperBlock (Pierce Biotechnology, Rockford, IL) for 60 min and incubated with rabbit polyclonal anti-GLUT1 or anti-IGF-IR ß-subunit antibodies (1:20,000; EMD Millipore, Cat# 07–1401, 04–298) in TBS containing 3% BSA for 120 min. Specificity was assured by absence of a signal following omission of the primary antibody.

Techniques: Expressing, Activity Assay

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Human placental GLUT1 glucose transporter expression and the fetal insulin-like growth factor axis in pregnancies complicated by diabetes

doi: 10.1016/j.bbadis.2019.06.002

Figure Lengend Snippet: Basal membrane GLUT1 and type 1 IGF receptors. Dependence of basal membrane GLUT1 expression on microvillous membrane expression of the type 1 IGF receptor ß subunit for control, GDM A2 and IDDM pregnancies. The regression line is for the control group only; no correlation was observed for the diabetic groups

Article Snippet: Membranes were then blocked with SuperBlock (Pierce Biotechnology, Rockford, IL) for 60 min and incubated with rabbit polyclonal anti-GLUT1 or anti-IGF-IR ß-subunit antibodies (1:20,000; EMD Millipore, Cat# 07–1401, 04–298) in TBS containing 3% BSA for 120 min. Specificity was assured by absence of a signal following omission of the primary antibody.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Expression of insulin-like growth factor I and its receptor in the liver of children with biopsy-proven NAFLD

doi: 10.1371/journal.pone.0201566

Figure Lengend Snippet: IGF-I (A) and IGF-IR (B) intensity/area (micronQ) in the different stages of fibrosis. Values are displayed as median and interquartile range (IQR).

Article Snippet: Sections were incubated over night at +4°C with anti-human: rabbit polyclonal anti-IGF-I antibody (dilution 1:300; Abcam, Cambridge, UK); rabbit polyclonal anti-IGF-IR antibody (dilution 1:50; Abcam, Cambridge, UK); mouse monoclonal anti-α-SMA antibody (dilution 1:100 Dako, Glostrup, Denmark).

Techniques:

Journal: PLoS ONE

Article Title: Expression of insulin-like growth factor I and its receptor in the liver of children with biopsy-proven NAFLD

doi: 10.1371/journal.pone.0201566

Figure Lengend Snippet: Correlation between IGF-I and IGF-IR expression, as intensity/area (micronQ) in the different stages of fibrosis.

Article Snippet: Sections were incubated over night at +4°C with anti-human: rabbit polyclonal anti-IGF-I antibody (dilution 1:300; Abcam, Cambridge, UK); rabbit polyclonal anti-IGF-IR antibody (dilution 1:50; Abcam, Cambridge, UK); mouse monoclonal anti-α-SMA antibody (dilution 1:100 Dako, Glostrup, Denmark).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Expression of insulin-like growth factor I and its receptor in the liver of children with biopsy-proven NAFLD

doi: 10.1371/journal.pone.0201566

Figure Lengend Snippet: Number of alpha-SMA positive cells (activated HSCs) expressing IGF-I (A) and IGF-IR (B) in the different stages of fibrosis. Values are displayed as median and interquartile range (IQR).

Article Snippet: Sections were incubated over night at +4°C with anti-human: rabbit polyclonal anti-IGF-I antibody (dilution 1:300; Abcam, Cambridge, UK); rabbit polyclonal anti-IGF-IR antibody (dilution 1:50; Abcam, Cambridge, UK); mouse monoclonal anti-α-SMA antibody (dilution 1:100 Dako, Glostrup, Denmark).

Techniques: Expressing

Journal: eLife

Article Title: IRS-1 acts as an endocytic regulator of IGF-I receptor to facilitate sustained IGF signaling

doi: 10.7554/eLife.32893

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-IGF-IRα , Santa Cruz Biotechnology , Santa Cruz Biotechnology: sc-712; RRID: AB_671788 , IB 1:1000.

Techniques: Transduction, Recombinant, Plasmid Preparation, Sequencing, Control, shRNA, Protease Inhibitor, Software